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The inventors have also found a DNA sequence which is a promoter region of a gene encoding a protein according to the invention. The present invention also relates to a DNA sequence comprising the promoter DNA sequence as defined above and a DNA sequence encoding a protein according to the present invention.

In the present invention, the inventors were initially obtained complementary DNA FDC by the steps of: - culture of lymphocyte cells called natural cytotoxic cells - isolating from these cell messenger RNA bound to the membranes of the intracellular endoplasmic reticulum - obtaining the single-stranded complementary DNA from the messenger RNA and the complementary double-stranded DNA - insertion into a vector such as bacteriophage lambda GT10 - preparing a single-stranded DNA probe from the messenger RNA of the cells and purification by a technique of hybridization-subtraction so as to select copies of RNA present in natural cytotoxic lymphocyte cells and absent in of other transformed hematopoietic cells.

Quando tiepido che potete lavorarlo, aggiungete un pò di farina e impastata bene deve diventare sodo ma non secco e lasciatelo coperto con sacchetto di nylon per circa 10 minuti. Siccome non volevo rischiare che i gnocchi si disfassero visto che era la prima volta che li facevo o pensato di utilizzare un uovo ma non potevo aggiungerlo nell'impasto bollente ed allora ho agito cosi.

Le mie amiche del gruppo Bloggalline bontà loro mi hanno aiutata a decidere cosa fare con un sacco di suggerimenti come : crespelle, gnocco fritto, tagliatelle, focaccini liguri, panelle siciliane, ecc.The extracellular portion of the LAG-3 protein corresponds to amino acids 1 to 420 of the LAG-3 protein described above.Comparing the sequence of the LAG-3 gene represented by c DNA FDC above as well as the organization exon / intron of the LAG-3 gene with other Ig / SF-like molecules revealed a close relationship the LAG-3 protein with the CD4 protein.The existence of this protein naturally on T cells has been demonstrated by: - preparation of sera raised against a synthetic peptide representing a region probably exposed to the outside of the FDC c DNA translation product having a protein structure looped, - immunoprecipitation of the LAG-3 protein by anti-peptide heteroantibodies.The proteins of the invention can further be obtained by other methods of purification of membrane proteins or conventional peptide synthesis or by application of genetic engineering techniques comprising inserting a DNA sequence encoding a protein according to the invention in an expression vector such as a plasmid and transformation of cells with this expression vector and culturing these cells.

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